Imaging and modelling of poly(3-hydroxybutyrate) synthesis in Paracoccus denitrificans

Abstract Poly(3-hydroxybutyrate) (PHB) granule formation in Paracoccus denitrificans Pd1222 was investigated by laser scanning confocal microscopy (LSCM) and gas chromatography analysis. Cells that had been starved for 2 days were free of PHB granules but resynthesized them within 30 min growth fresh medium with succinate. In most cases, the distributed randomly, although some cases they appeared a more organized pattern. The rates accumulation analyzed frame Genome-Scale Metabolic Model (GSMM) containing 781 metabolic genes, 1403 reactions 1503 metabolites. model used to obtain quantitative predictions biomass yields synthesis during aerobic on succinate as sole carbon energy sources. results revealed an initial fast stage accumulation, which all acetyl-CoA originating from diverted production. next characterized tenfold lower production rate simultaneous onset exponential growth, predominantly drained into TCA cycle. Previous research has shown correlates cytosolic concentration. It also is not transcriptionally regulated. Our are consistent mentioned findings suggest that, absence cell cellular channeled synthesis, while it cycle, causing reduction pool concomitant decrease acetoacetyl-CoA (the precursor synthesis).